![]() The pre-cut membranes and filters include 20 sheets of polyvinylidene difluoride (PVDF) or nitrocellulose and 80 sheets of absorbent blotting paper (0.85 mm thickness). Invitrogen Power Blotter Pre-cut Membranes and Filters are designed for the transfer of proteins using the Power Blotter System. The protein binding capacity is 209 µg/cm 2. The proteins bind to the membrane through hydrophobic and electrostatic interactions. The membrane is compatible with commonly used detection methods such as staining, chemiluminescence, and fluorescence. The nitrocellulose membrane (0.2 µm pore size) in the stack is composed of 100% pure nitrocellulose to provide high-quality transfer. This membrane has lower background levels and increased sensitivity for fluorescent probing than regular PVDF or nitrocellulose membranes. ![]() The proteins bind to the membrane through hydrophobic interactions. The membrane is compatible with commonly used detection methods such as staining, immunodetection, and fluorescence. The PVDF membrane (0.45 µm pore size, low fluorescence) has higher binding capacity than nitrocellulose. Each Power Blotter Select Transfer Stack contains filter paper, membrane, and the appropriate cathode and anode buffers incorporated into a solid gel matrix to allow fast, reliable transfer of proteins. They are disposable, pre-assembled stacks that come with an integrated preactivated PVDF or nitrocellulose transfer membrane for dry blotting of proteins from polyacrylamide gels. ![]() The Invitrogen Power Blotter Select Transfer Stacks are used to transfer proteins using the Power Blotter System. The regular size (13 cm x 8.3 cm) can be used for all Invitrogen midi gels (or other midi gel types), or two Invitrogen mini gels (or other mini gel types), and E-PAGE 48 or 96 gels. The mini size (8 cm x 8 cm) can be used for all Invitrogen precast and handcast mini gels (or other mini gel types). The protein binding capacity is 240 µg/cm 2. The proteins bind to the membrane due to hydrophobic interactions. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. The PVDF membrane is preactivated and ready for use without any pretreatment with alcohols. The PVDF membrane (0.2 µm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. The proteins bind to the membrane due to hydrophobic and electrostatic interactions. ![]() The nitrocellulose membrane (0.2 µm pore size) in this stack is composed of 100% pure nitrocellulose to provide high-quality transfer. Each transfer stack contains a copper coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins. IBlot and iBlot 2 Transfer Stacks are consumable stacks with integrated PVDF or nitrocellulose transfer membranes for dry blotting of proteins. ![]()
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